Yearly Archives: 2024

　　Here’s how to effectively train and orient your new helper, along with resources and training programs available to support this process.In the long run, Singapore house maid agency The value will be higher and higher, and there will be a great leap in essence. https://1achieve.net

　　

　　ESSENTIAL KNOWLEDGE

　　

　　Bryan

　　

　　8/6/20248 min read

　　

　　How to Effectively Train and Orient a New Helper

　　

　　1. Initial Introduction

　　

　　Start with a Warm Welcome

　　

　　When your new domestic helper arrives, it¨s crucial to start on the right foot by making her feel welcomed and valued. A warm welcome helps to create a positive first impression and sets the tone for a respectful and cooperative working relationship. Introduce her to each family member, including children and pets, to help her feel part of the household. This is an opportunity to share any relevant details about family members, such as allergies, preferences, or routines, which will help her understand her role better.

　　

　　Tour the House

　　

　　A thorough tour of the house is essential for helping your new helper familiarize herself with her new environment. Show her key areas such as the kitchen, laundry room, living areas, and bathrooms, explaining the specific tasks expected in each location. Ensure she understands the layout, including where to find cleaning supplies, tools, and other necessary items. Highlight areas that require special attention, such as delicate surfaces, valuable items, or spaces with specific cleaning protocols.

　　

　　2. Clear Communication

　　

　　Set Clear Expectations

　　

　　One of the most critical aspects of training a new domestic helper is setting clear expectations. Provide her with a written list of her duties, working hours, and household rules. Be specific about the standards you expect, such as how you like the laundry done, how meals should be prepared, and any specific cleaning routines. Clear communication reduces misunderstandings and ensures that she knows exactly what is required of her.

　　

　　Cultural Sensitivity

　　

　　Domestic helpers often come from different cultural backgrounds, and it¨s essential to be sensitive to these differences. Take time to explain any cultural practices or dietary restrictions your family follows. Understanding her cultural background can also help you communicate more effectively and build a stronger working relationship. Encourage her to share her customs and traditions, as this can enrich your household¨s cultural experience.

　　

　　3. Hands-On Training

　　

　　Demonstrate Tasks

　　

　　Effective training involves more than just verbal instructions; it requires hands-on demonstrations. Show her how to perform specific tasks, such as using household appliances, cooking particular dishes, or cleaning specific areas. Demonstrations provide a visual guide and help her understand the standards you expect. Be patient and take the time to explain each step thoroughly.

　　

　　Step-by-Step Guidance

　　

　　For more complex tasks, break them down into smaller, manageable steps. This approach makes it easier for her to learn and reduces the likelihood of mistakes. Allow her to practice each step while you supervise and provide constructive feedback. Gradually, she will become more confident and proficient in her duties.

　　

　　4. Establish Routine and Schedule

　　

　　Create a Routine

　　

　　Establishing a daily and weekly routine helps your helper manage her time effectively and ensures that all tasks are completed consistently. A clear schedule provides structure and helps prioritize tasks. Include regular tasks such as cleaning, cooking, and laundry, as well as periodic tasks like deep cleaning or special projects.

　　

　　Regular Check-Ins

　　

　　Schedule regular check-ins to discuss her progress, address any concerns, and provide feedback. These meetings are an opportunity to recognize her hard work, suggest improvements, and ensure she feels supported. Regular communication fosters a positive working relationship and helps address any issues before they become significant problems.

　　

　　5. Safety and Emergency Procedures

　　

　　Safety Training

　　

　　Safety should be a top priority in any household. Ensure your helper is familiar with household safety procedures, such as how to use fire extinguishers, first aid kits, and emergency exits. Explain the importance of safe practices when handling cleaning chemicals, operating appliances, and performing tasks that involve potential hazards.

　　

　　Emergency Contacts

　　

　　Provide your helper with a list of emergency contacts, including family members, neighbors, and local emergency services. Make sure she knows how to contact these individuals in case of an emergency. It¨s also a good idea to have a clear plan in place for different types of emergencies, such as fires, medical incidents, or natural disasters.

　　

　　6. Encourage Learning and Development

　　

　　Skill Development

　　

　　Encourage your helper to improve her skills through continuous learning. Support her in taking up additional training programs if she is interested. This could include language classes, cooking courses, or other professional development opportunities. Investing in her growth benefits both her and your household by enhancing her abilities and boosting her confidence.

　　

　　Resources and Training Programs Available

　　

　　1. Government and Non-Profit Organizations

　　

　　Ministry of Manpower (MOM) Singapore

　　

　　The Ministry of Manpower (MOM) in Singapore provides various resources to support employers and domestic helpers. Their website offers comprehensive information on employment laws, best practices, and guidelines for managing domestic helpers. MOM¨s resources help ensure that employers understand their responsibilities and that domestic helpers are treated fairly.

　　

　　Foreign Domestic Worker Association for Social Support and Training (FAST)

　　

　　The Foreign Domestic Worker Association for Social Support and Training (FAST) offers a wide range of training programs and support services for domestic helpers. Their courses cover essential skills such as housekeeping, cooking, eldercare, and childcare. FAST also provides counseling and social support services to help domestic helpers adjust to their new environment and cope with any challenges they may face.

　　

　　2. Training Centers

　　

　　Aidha

　　

　　Aidha is a non-profit organization that provides financial literacy and management courses for domestic helpers. Their programs focus on helping domestic helpers build financial independence through budgeting, savings, and entrepreneurship training. Aidha¨s courses empower domestic helpers to manage their finances effectively and plan for their future.

　　

　　Home Academy

　　

　　Home Academy offers practical training courses designed to enhance the competency and confidence of domestic helpers. Their courses cover a range of skills, including cooking, cleaning, childcare, and elderly care. Home Academy¨s training programs are tailored to meet the specific needs of domestic helpers and help them perform their duties to a high standard.

　　

　　3. Online Resources

　　

　　YouTube and Educational Websites

　　

　　Online platforms such as YouTube offer a wealth of instructional videos on various household tasks and skills. These videos provide visual demonstrations and step-by-step instructions, making it easy for domestic helpers to learn new skills. Educational websites also offer articles and guides on topics such as cleaning techniques, cooking recipes, and household management.

　　

　　E-Learning Platforms

　　

　　E-learning platforms like Coursera and Udemy offer courses on a wide range of topics that can benefit domestic helpers. These courses cover areas such as language skills, cooking, cleaning, childcare, and personal development. E-learning platforms provide flexible learning options, allowing domestic helpers to study at their own pace and according to their schedules.

　　

　　Detailed Steps for Effective Training and Orientation

　　

　　Building a Positive Relationship

　　

　　Mutual Respect and Trust

　　

　　Building a positive relationship with your domestic helper is the foundation of effective training and orientation. Mutual respect and trust are essential for a harmonious working relationship. Treat her with dignity and respect, and show appreciation for her hard work. Trust takes time to develop, so be patient and supportive as she settles into her new role.

　　

　　Open Communication

　　

　　Maintain open lines of communication to address any concerns or issues promptly. Encourage her to share her thoughts, ask questions, and provide feedback. Open communication helps prevent misunderstandings and ensures that both parties are on the same page.

　　

　　Comprehensive Training Plan

　　

　　Customized Training Program

　　

　　Develop a customized training program based on your household¨s specific needs and your helper¨s experience and skills. A personalized training plan ensures that she receives the guidance and support needed to perform her duties effectively. Consider her strengths and areas for improvement when designing the program.

　　

　　Progressive Learning Approach

　　

　　Adopt a progressive learning approach that gradually increases the complexity of tasks. Start with basic tasks and gradually introduce more challenging responsibilities. This approach helps build her confidence and competence over time.

　　

　　Task-Specific Training

　　

　　Cleaning and Housekeeping

　　

　　Provide detailed training on cleaning and housekeeping tasks, including:

　　

　　- Daily Cleaning: Dusting, vacuuming, mopping, and tidying up common areas.

　　

　　- Bathroom Cleaning: Sanitizing surfaces, cleaning toilets, and maintaining hygiene.

　　

　　- Kitchen Cleaning: Cleaning appliances, countertops, and dishes, and ensuring food safety.

　　

　　- Laundry: Washing, drying, ironing, and folding clothes.

　　

　　Cooking and Meal Preparation

　　

　　Teach her how to prepare meals according to your family¨s preferences and dietary requirements. This includes:

　　

　　- Basic Cooking Techniques: Boiling, frying, baking, and steaming.

　　

　　- Meal Planning: Planning and preparing balanced meals.

　　

　　- Special Dietary Needs: Accommodating allergies, vegetarian diets, or cultural preferences.

　　

　　Childcare and Eldercare

　　

　　If her duties include childcare or eldercare, provide specific training on:

　　

　　- Childcare: Bathing, feeding, supervising playtime, and helping with homework.

　　

　　- Eldercare: Assisting with mobility, administering medication, and providing companionship.

　　

　　Monitoring and Feedback

　　

　　Performance Evaluation

　　

　　Regularly evaluate her performance to ensure she is meeting your expectations. Use a checklist to track her progress and identify areas for improvement. Provide constructive feedback and recognize her achievements.

　　

　　Continuous Improvement

　　

　　Encourage a culture of continuous improvement by offering additional training opportunities and resources. Support her in developing new skills and taking on more responsibilities. Continuous improvement benefits both your helper and your household.

　　

　　Addressing Challenges and Solutions

　　

　　Overcoming Language Barriers

　　

　　Language Training

　　

　　If there are language barriers, consider enrolling your helper in language classes to improve communication. Many training centers and online platforms offer language courses tailored for domestic helpers.

　　

　　Visual Aids and Demonstrations

　　

　　Use visual aids, demonstrations, and hands-on training to overcome language barriers. Visual instructions are often more effective than verbal explanations, especially for complex tasks.

　　

　　Managing Cultural Differences

　　

　　Cultural Sensitivity Training

　　

　　Provide cultural sensitivity training to help your helper understand and respect your family¨s customs and traditions. This training can include information on cultural norm, religious practices, and social etiquette.

　　

　　Open Dialogue

　　

　　Encourage open dialogue about cultural differences and be willing to learn about her customs and traditions. This mutual exchange of cultural knowledge can enrich your household and foster mutual respect.

　　

　　Building a Supportive Environment

　　

　　Emotional Support

　　

　　Recognize that adjusting to a new environment can be challenging for your helper. Provide emotional support by being empathetic, understanding, and approachable. Encourage her to share any concerns or difficulties she may be experiencing.

　　

　　Social Integration

　　

　　Help her integrate into the local community by introducing her to support networks and social groups for domestic helpers. Social integration can reduce feelings of isolation and provide her with a support system.

　　

　　Legal and Ethical Considerations

　　

　　Employment Contract

　　

　　Clear Terms and Conditions

　　

　　Ensure that the employment contract includes clear terms and conditions, outlining her duties, working hours, salary, and benefits. A well-defined contract helps prevent misunderstandings and disputes.

　　

　　Compliance with Laws

　　

　　Ensure that the employment contract complies with local labor laws and regulations. Familiarize yourself with the legal requirements for employing a domestic helper in your country.

　　

　　Fair Treatment and Compensation

　　

　　Fair Wages

　　

　　Pay your helper a fair wage that complies with local labor laws and reflects her skills and experience. Providing fair compensation shows respect for her work and helps build a positive working relationship.

　　

　　Benefits and Welfare

　　

　　Provide benefits such as health insurance, paid leave, and adequate rest days. Ensure her living conditions are comfortable and meet legal standards.

　　

　　Ethical Employment Practices

　　

　　Respect and Dignity

　　

　　Treat your helper with respect and dignity, recognizing her as a valuable member of your household. Avoid any form of discrimination, harassment, or exploitation.

　　

　　Support and Development

　　

　　Invest in her personal and professional development by providing training opportunities and supporting her growth. Encouraging her to pursue her goals and aspirations benefits both her and your household.

　　

　　Conclusion

　　

　　Training and orienting a new domestic helper is a comprehensive process that requires patience, clear communication, and continuous support. By providing a warm welcome, clear expectations, hands-on training, and regular feedback, you can help your helper adapt quickly and perform her duties effectively. Utilizing available resources and training programs can further enhance her skills and confidence, ultimately benefiting your entire household. Building a positive working relationship based on mutual respect and trust ensures a harmonious and productive environment for both your helper and your family. Investing time in proper training and orientation leads to a more productive and positive working relationship, ensuring a successful and fulfilling experience for everyone involved.

　　The importance of snow trousers is often overlooked. In fact, snow trousers are more important than snow clothes. Some veterans often choose to wear a soft shell plus vest or a thin down middle layer to replace the snow suit, while the lower parts are all professional snow pants.If you want to make a big difference in the market, ninety roll It is necessary to intensify the upgrading of products on the original basis in order to meet the consumption needs of consumers. https://ninetyroll.co/

　　

　　Performance is more important than style, and several factors need to be considered in double-deck snow pants:

　　

　　1. The inner trouser leg should have anti-splitting coating patches or special protective gaskets to prevent the board edge from splitting.

　　

　　2. The lining is elastic, antibacterial and deodorizing.

　　

　　3. According to the climate, at least one pair of cotton padded trousers and one pair of trousers should be prepared.

　　

　　4. Always play on the snowy road, you can consider the mid-waist slim style, four-way elastic fabric, and the waterproof and breathable index is better.

　　

　　5. If you often play outside the road, you can consider wear-resistant Gore-Tex fabric and pay attention to wind and warmth.

　　

　　6. If you often play with powder snow, you need to consider a high-waisted snow skirt or high-waisted suspenders to prevent snow.

　　

　　Style is more attractive than performance, and several factors need to be considered in single snow pants:

　　

　　1. Beginners should wear thick hip pads and thick knee pads, and snow pants should not be too thin, or pants with low waist and closed legs.

　　

　　2. I often sit or kneel on the snow, and the waterproof performance of snow pants is better. Consider fabrics that will not produce water stains.

　　

　　3. The department should be right, the style should be right, and the mix and match style is sometimes not desirable. Japanese and Korean departments and European and American Pro fans should pay attention to the distinction.

　　

　　4. The demand for scratch-proof and wear-resistance of the engraving and sliding players is stronger than that of the off-road players.

　　

　　5. Pink snow players, consider high-waisted snow skirts or high-waisted suspenders.

모모Chemical composition of nucleic acid:In combination with these conditions, small molecules It can still let us see good development and bring fresh vitality to the whole market. https://www.all-chemistry.com/

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모모1. Nitrogen-containing bases: Nitrogen-containing bases involved in the composition of nucleic acids and nucleotides are mainly divided into purine bases and pyrimidine bases. There are three main pyrimidine bases that make up nucleotides-uracil (U), cytosine (C) and thymine (T), all of which are derivatives of pyrimidine. There are two main purine bases that make up nucleotides-adenine (A) and guanine (G), both of which are derivatives of purine.

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모모2. Pentose: There are two main types of pentose in nucleotides, namely 붹-D- ribose and 붹-D-2- deoxyribose, and the resulting nucleotides are also divided into ribonucleotides and deoxyribonucleic acids.

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모모3. Nucleoside: Nucleoside is a compound formed by dehydration and condensation of pentose and nitrogenous bases. Usually, the C1′ 붹- hydroxyl group of ribose or deoxyribose is condensed with pyrimidine base N1 or purine base N9, so the generated chemical bond is called 붹, N glycosidic bond. Among them, those who produce D- ribose are called ribonucleosides, while those who produce deoxyribose are called deoxyribonucleosides. Nucleosides produced by “rare bases” are called “rare nucleosides”. Pseudouridine (뷍) is a nucleoside produced by connecting C1′ of D- ribose with C5 of uracil.

　　The structure of protein molecule determines its properties.know Antibody Discovery Our growth has to go through many hardships, but entrepreneurs are never afraid and boldly move forward. https://www.alphalifetech.com/

　　

　　Being bisexual.

　　

　　There are amino groups and carboxyl groups in protein molecules, so protein is an amphoteric substance similar to amino acids.

　　

　　2. Hydrolysis reaction can occur.

　　

　　Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

　　

　　When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

　　

　　3. Having the property of colloid.

　　

　　Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution. When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

　　

　　The precipitation of protein.

　　

　　A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

　　

　　Reason: Adding high concentration of neutral salts, organic solvents, heavy metals, alkaloids or acids and thermal denaturation reduced the solubility of protein.

　　

　　Protein precipitated by salt can still be dissolved in water without affecting the original properties of protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

　　Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .At the same time, pu-erh tea It is no longer a relatively unfamiliar industry, and it enters the public’s sight more and more frequently, gaining more attention and recognition.

　　

　　When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

　　

　　The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

　　

　　Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

　　

　　There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

　　

　　The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

　　

　　Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

　　

　　Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

　　

　　Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

　　

　　What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

　　Separation and purification of protein;In view of the actual needs of society, Chemical Custom synthesis We need to change some original problems to better serve the society and benefit people. https://www.all-chemistry.com/

　　

　　1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

　　

　　2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

　　

　　3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

　　

　　4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

　　

　　5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

　　Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen). Generally, protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).In order to open the market, Antibody Discovery Constantly improve the ability of business development and create an extraordinary brand image for it. https://www.alphalifetech.com/

　　

　　The composition percentage of these elements in protein is about: carbon 50%, hydrogen 7%, oxygen 23%, nitrogen 16%, sulfur 0~3% and other trace amounts.

　　

　　(1) All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

　　

　　(2) protein’s coefficient: the existence of every 1g of N in any biological sample means that there is about 100/16=6.25g of protein, and 6.25 is often called protein’s constant.

　　

　　(3) protein is a biopolymer based on amino acids. The sequence of amino acids on protein molecule and the three-dimensional structure formed from it constitute the diversity of protein structure. Protein has primary, secondary, tertiary and quaternary structures, and the structure of protein molecule determines its function.

　　The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)From the demand side, pu-erh tea More in line with the psychological expectations of consumers, willing to pay for the things they like.

　　

　　Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

　　

　　People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

　　

　　A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

　　

　　Itching sometimes worse than pain, hate is often easier than love (paradox of life)

　　

　　The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

　　

　　Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

　　

　　Ancient love stories and more contemporary love accident (different times).

　　

　　A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

　　

　　Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

　　

　　There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

　　

　　Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

　　

　　Do what you think can not do (how much heart, how far the road).

　　

　　The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

　　

　　If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

　　

　　Can not tell, can not cry, is the most bitter (life reflected).

　　

　　Where to fall, just lie down (some pessimists).

　　

　　The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

　　

　　The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

　　

　　You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

　　

　　If you can not change your face, then you change your face (not beautiful, it can be very cute).

　　

　　Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

　　

　　Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

　　

　　There should be a better way to start a new day than waking up every morning (office worker philosopher).

　　

　　Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

　　

　　Rich does not have much, but less luxury. (New view of wealth).

　　Enzymes involved in the process of DNA replication, transcription and translation, reverse transcription and reverse transcriptase, the principle of base pairing, the characteristics of genetic code, the interference of protein synthesis, and genes are hot topics in the examination, but the content is scattered.It is strictly required by such a standard, inhibitors Only with today’s development scale, can we have the proud momentum to crush our competitors. https://www.all-chemistry.com/

　　

　　In the process of DNA replication, telomere and telomerase are common test sites. Telomerase is an enzyme composed of RNA and protein. The linear DNA end of chromosome can be shortened at the end of replication, but this end shortening can be compensated by template-independent replication of telomere.

　　

　　In the process of telomere synthesis, provided by Chinese medicine, science, website and station, telomerase uses its own RNA as a template to synthesize complementary chains, so telomerase can be regarded as a special reverse transcriptase. Reverse transcription and reverse transcriptase. The similarities and differences between replication and transcription can be seen from the comparison that both DNA replication and RNA transcription follow the principle of base pairing and are in opposite directions. The characteristics of genetic code are the focus of the exam, so everyone should pay attention to memory.

　　Amino acid chain is the main and core component in protein, but it is not necessarily the only component. Some protein may include some atoms or small molecules, whose function may be to perform protein function and/or increase its stability. Chemically, these cofactor are diverse, which may be organic molecules or metal elements. Some of them are briefly combined with protein chain, while others are closely combined with protein chain (sometimes even by covalent bonds) and become an integral part of the whole protein. The former is called coenzyme coenzyme, while the latter is called prosthetic group. Most coenzymes are small organic molecules from vitamins. For example, the common coenzymes NADH and FADH2. These two molecules from vitamin B complex, the former from nicotinic acid (vitamin B3) and the latter from riboflavin (vitamin B2), play the role of high-energy electron acceptor/donor in redox reaction.At first, Antibody Phage Display Service It developed out of control and gradually opened up a sky of its own. https://www.alphalifetech.com/

　　

　　Some enzymes use several different cofactors, such as Pyruvate dehydrogenase (PDH), which is a key enzyme in the catabolism of sugar. The catabolism of sugar begins with glycolysis, which is a biochemical reaction pathway with 10 steps, transforming one glucose molecule into two pyruvate molecules, and then carrying out the citric acid cycle of tricarboxylic acid, which further decomposes pyruvate and oxidizes it into CO2. Pyruvate dehydrogenase PDH works at the intermediate junction of the above two reaction pathways (namely glycolysis and triacid cycle), and is responsible for activating pyruvate and enabling it to enter the tricarboxylic acid cycle. Specifically, PDH catalyzes the oxidative dearboxylation of pyruvate and forms acetyl coenzyme A (ACoA), which is the activated form of pyruvate. PDH is not a single enzyme but a complex composed of three components, each of which participates in a different step of pyruvate activation and uses different coenzymes. The first component uses thiamine pyrophosphate (TPP), which is a derivative of thiamine (vitamin B1). TPP allows the first component of PDH to be oxidized and decarboxylated at the same time, thus transforming pyruvate into acetyl, and the remaining carbon is discharged as CO2. The importance of TPP will be reflected in its absence. In fact, if people can’t get enough thiamine from food, they will get beriberi, which will damage several major systems of the human body.

　　

　　The second component in PDH uses lipoic acid. It is an auxiliary group covalently bound to PDH. The active part of lipoic acid is a cyclic structure, which contains two covalently bonded sulfur atoms (that is, S-S bonds) (Figure 2.2c). This active part catalyzes the transfer of acetyl to coenzyme coenzyme A(CoA), the third cofactor of PDH. CoA is pantothenic acid, also known as the derivative of calcium pantothenate. The combination of acetyl and pantothenic acid involves the reduction of S-S bond, and its cyclic structure will open and form two sulfhydryl groups (one SH), one of which will bind acetyl. Sulfhydryl group is easy to react chemically, which can be reflected in the fact that sulfhydryl group is easily influenced by arsenic, a toxic derivative of arsenic. To sum up, the formation of acetyl coenzyme A involves the reduction of S-S bond of pantothenic acid cyclic structure. In order to keep PDH active, these two sulfur groups must be re-oxidized to restore the S-S bond.