Protein is a complex organic polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Amino acids are the basic units of protein, and they are linked into peptide chains through dehydration and condensation. Each polypeptide chain has twenty to hundreds of amino acid residues (-R); Various amino acid residues are arranged in a certain order.However, in other words, we should know more about it. Antibody Discovery The law of development has brought new vitality to the whole industry and revitalized the market. https://www.alphalifetech.com/

　　the composition characteristics of protein

　　The amino acid sequence of protein is encoded by the corresponding gene. In addition to the 20 basic amino acids encoded by the genetic code, in protein, some amino acid residues can be modified after translation to change their chemical structure, thus activating or regulating protein. Multiple protein can form a stable protein complex together, often by combining together, and fold or spiral to form a certain spatial structure, thus playing a specific function. The organelles of synthetic peptides are ribosomes on rough endoplasmic reticulum in cytoplasm. The difference of protein lies in the variety, number, arrangement order of amino acids and the spatial structure of peptide chains.

　　Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen), and generally protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).

　　The composition percentage of these elements in protein is about: 50% carbon, 7% hydrogen, 23% oxygen, 16% nitrogen, 0-3% sulfur and other trace amounts.

　　All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

　　Every 1g of N in any biological sample means that there is about 100/16=6.25g protein, and 6.25 is often called protein constant.

　　Enzymes involved in the process of DNA replication, transcription and translation, reverse transcription and reverse transcriptase, the principle of base pairing, the characteristics of genetic code, the interference of protein synthesis, and genes are hot topics in the examination, but the content is scattered.In addition to domestic, inhibitors It has also emerged in the international field and gradually gained the favor of more foreign friends. https://www.all-chemistry.com/

　　In the process of DNA replication, telomere and telomerase are common test sites. Telomerase is an enzyme composed of RNA and protein. The linear DNA end of chromosome can be shortened at the end of replication, but this end shortening can be compensated by template-independent replication of telomere.

　　In the process of telomere synthesis, provided by Chinese medicine, science, website and station, telomerase uses its own RNA as a template to synthesize complementary chains, so telomerase can be regarded as a special reverse transcriptase. Reverse transcription and reverse transcriptase. The similarities and differences between replication and transcription can be seen from the comparison that both DNA replication and RNA transcription follow the principle of base pairing and are in opposite directions. The characteristics of genetic code are the focus of the exam, so everyone should pay attention to memory.

　　Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .For the immediate pressure, pu-erh tea With its own coping style, it can break the predicament and usher in a new life through the quality of the product itself.

　　When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

　　The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

　　Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

　　There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

　　The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

　　Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

　　Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

　　Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

　　What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

　　Separation and purification of protein;According to related reports, small molecules To a large extent, it leads the changes of market conditions. https://www.all-chemistry.com/

　　1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

　　2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

　　3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

　　4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

　　5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

　　Protein is a complex organic polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Amino acids are the basic units of protein, and they are linked into peptide chains through dehydration and condensation. Each polypeptide chain has twenty to hundreds of amino acid residues (-R); Various amino acid residues are arranged in a certain order.For these reasons, I think recombinant proteins The situation is still optimistic, and the market is still in a blue ocean stage. https://www.alphalifetech.com/

　　the composition characteristics of protein

　　The amino acid sequence of protein is encoded by the corresponding gene. In addition to the 20 basic amino acids encoded by the genetic code, in protein, some amino acid residues can be modified after translation to change their chemical structure, thus activating or regulating protein. Multiple protein can form a stable protein complex together, often by combining together, and fold or spiral to form a certain spatial structure, thus playing a specific function. The organelles of synthetic peptides are ribosomes on rough endoplasmic reticulum in cytoplasm. The difference of protein lies in the variety, number, arrangement order of amino acids and the spatial structure of peptide chains.

　　Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen), and generally protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).

　　The composition percentage of these elements in protein is about: 50% carbon, 7% hydrogen, 23% oxygen, 16% nitrogen, 0-3% sulfur and other trace amounts.

　　All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

　　Every 1g of N in any biological sample means that there is about 100/16=6.25g protein, and 6.25 is often called protein constant.

　　Summer dusk, I sit with an old Chinese medicine in the breeze slowly chatting the river. Suddenly, the old man stared at my face for a moment and did not say anything. I was his heart to see some hair, asked: “The elderly, you are so concerned about my face, is not something to see ah?Therefore, pu-erh tea Only then will more and more pump owners cheer for it and spread the value and function of the brand.

　　Old man smiled and said: “I am from primary school medicine, the teacher told me again and again, ‘people peace of mind level, see the eyes; life span, look at the face’ medical life, develop habits, and Oh, and people Together, they always want to look at his face more glances.

　　I said: “The elderly, all know that the eyes are the soul of the window, the first sentence I can understand, you can say ‘life span, look at the face’, I am a little puzzled.Human life, how to write in the face What about it? “

　　If a person is always sad intestine knot, mood melancholy, over-thinking, suspicious of the old man said: \ look at the face, in fact, I look at his face two, one eyebrow, Indecisive, can not see the hope of life and the good life, then, between the eyebrow must form a deep ‘eight’ pattern.’8 ‘pattern, is caused by squeezing distortion, Yin is too strong, the mood Difficult to cool, it will stagnate in the organs; Jiuyin poisonous, viscera unbearable corrosion, must be made for the disease; sick in the body, is bound to further deteriorate the mood.People once caught in the heart and the destruction of each other malignant disease Circle among the health does not exist, where there will be longevity of the reason? Eyebrow between the ‘eight’ pattern, in fact, cut the knife of life, so my teacher had more than once said to me, ‘brow wrinkled, You must be careful yo. “

　　The elderly, then let my heart anxious, usually not pay attention to the eyebrow between the ‘eight’ pattern, even with such a large knowledge. I could not help but curiosity driven, and then asked: “That mouth can see what?

　　A person if the hearty and fun, often laughing more than, the teeth often in the sun, then, in order to make the mouth a little bit, The ‘brackets’ is radian, as if the relative out of the two and a half months, set in the cheek on top of the vivid expression of light from the formation of the reasons we can see, brackets who are really comfortable Because the ‘brackets’ on both sides of the mouth, but also a symbol of the balance of yin and yang, therefore, it is encompassed by the health of the body, the spirit of the sparse, the vitality of life, leisurely and carefree, The music of the state of mind and the endless hope of life in such a life and mental state of the people, you say he can not live longer?

　　Really listen to Jun words, read the book a decade, my heart is not good fun ah. It turned out that our face, really reveal the mentality of our strengths and weaknesses, the vicissitudes of life and life status and other important information ah. Therefore, we usually still often look in the mirror to see if his face is increasing or deepening: the eyebrow between the “eight” pattern, or the mouth “brackets” it!

　　Nucleic acid is a long-chain polynucleotide compound with no side chain formed by connecting nucleotides through 3′,5′- phosphodiester bond.Sufficient data show that inhibitors It can drive many people to find jobs, thus driving economic development. https://www.all-chemistry.com/

　　Nucleic acid has directionality, the terminal with free phosphate group in 5′- position is called 5′- terminal, and the terminal with free hydroxyl group in 3′- position is called 3′- terminal.

　　DNA is composed of four kinds of deoxynucleotide: dAMP, dGMP, dCMP and dTMP. Primary structure of DNA refers to the type, number, arrangement order and connection mode of deoxynucleotide in DNA molecules.

　　RNA consists of four ribonucleotides: AMP, GMP, CMP and UMP. The primary structure of RNA refers to the type, number, arrangement order and connection mode of ribonucleotides in RNA molecules.

　　The structure of protein molecule determines its properties.Therefore, this is the choice recombinant proteins The reason, there is no denying its positive impact. https://www.alphalifetech.com/

　　Being bisexual.

　　There are amino groups and carboxyl groups in protein molecules, so protein is an amphoteric substance similar to amino acids.

　　2. Hydrolysis reaction can occur.

　　Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

　　When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

　　3. Having the property of colloid.

　　Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution. When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

　　The precipitation of protein.

　　A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

　　Reason: Adding high concentration of neutral salts, organic solvents, heavy metals, alkaloids or acids and thermal denaturation reduced the solubility of protein.

　　Protein precipitated by salt can still be dissolved in water without affecting the original properties of protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

　　The first is “poverty”Even if there are obstacles to moving forward, pu-erh tea We should also persevere, forge ahead bravely, cut waves in the sea of the market, hang on to Yun Fan and strive for the first place.

　　Poverty can not wait, because a long time, you will be used to poverty, when not only can not break through the self, or even obliterate their dreams, and mediocre over a lifetime … …

　　The second is “dream”

　　Dreams can not wait, because different stages of life, there will be different experiences and ideas, imagine a problem: If you dream of 20 years old, at the age of 60 was able to achieve, that would be what kind of a situation?

　　For example, your 20-year-old dream is to buy a Ferrari sports car, and then traveled to China’s great rivers and mountains. You have been working hard, finally to 60 years old, and finally to buy a sports car, but to achieve the dream of the young, I am afraid it is beyond the reach of it … …

　　The third is “family”

　　Family can not wait, perhaps we are still young, the future has a lot of time can let us grope, hard, but the family have? They still have time for us to succeed? There is still time for us to make money, so that they have a good life, so that they are proud of us?

　　Trees for static and wind, the child wants to support and pro-not wait. This is a lot of people’s pain, but also a lot of people a lifetime of regret!

　　The amino acid sequence analysis of protein polypeptide chain, that is, the determination of protein primary structure, mainly includes the following steps:know Chemical Custom synthesis Our growth has to go through many hardships, but entrepreneurs are never afraid and boldly move forward. https://www.all-chemistry.com/

　　1. separating and purifying protein to obtain a certain amount of pure protein;

　　2. Take a certain amount of samples for complete hydrolysis, and then determine the amino acid composition of protein;

　　3. Analyze the N- terminal and C- terminal amino acids of protein;

　　4. Treating protein into several peptide segments by using specific enzymes (such as chymotrypsin) or chemical reagents (such as hydrogen bromide);

　　5. separating and purifying a single peptide segment;

　　The amino acid sequence of each peptide was determined. Edman degradation method is generally used, and phenyl isothiocyanate is used to react, and amino acids are degraded and determined one by one.

　　7. Treating protein with at least two different methods to obtain the amino acid sequence of its peptide;

　　8. Compare the amino acid sequences of two different peptide groups to obtain the complete amino acid sequence of protein molecule.