This comprehensive guide will walk you through the step-by-step process of hiring a domestic helper, including the necessary documentation and legal requirements.As it happens, many people are killed maid services Singapore And bring more benefits, make it flourish, and promote the industry greatly. https://1achieve.net

　　

　　ESSENTIAL KNOWLEDGE

　　

　　7/24/20246 min read

　　

　　Domestic maid in modern living room in Singapore

　　

　　Step-by-Step Guide on How to Hire a Domestic Helper

　　

　　1. Determine Your Needs

　　

　　The first step in hiring a domestic helper is to assess your household’s specific needs. Determine what tasks the helper will be responsible for, such as:

　　

　　- Cleaning: General housekeeping duties including vacuuming, mopping, dusting, and laundry.

　　

　　- Cooking: Meal preparation and kitchen duties, which may also include grocery shopping.

　　

　　- Childcare: Taking care of children, including feeding, bathing, and supervising their activities.

　　

　　- Elderly Care: Assisting elderly family members with daily tasks, administering medication, and ensuring their well-being.

　　

　　- Pet Care: Looking after pets, which may include feeding, walking, and grooming.

　　

　　Understanding your specific needs will help you select the right candidate who possesses the skills and experience to fulfill these tasks.

　　

　　2. Budgeting and Salary

　　

　　Establishing a budget is crucial before embarking on the hiring process. The monthly salary of a domestic helper in Singapore varies based on factors such as the helper¨s experience and nationality. On average, salaries range from SGD 450 to SGD 600.

　　

　　In addition to the salary, consider other expenses such as:

　　

　　- Food and Accommodation: Helpers are entitled to adequate food and a suitable living space.

　　

　　- Medical Insurance: Mandatory coverage of at least SGD 15,000 per year for inpatient care and day surgery.

　　

　　- Levy: Monthly levy payment to the Ministry of Manpower (MOM). Rates vary depending on the household¨s needs.

　　

　　- Security Bond: A financial guarantee to the Singapore government, typically SGD 5,000 for non-Malaysian helpers.

　　

　　3. Selecting an Employment Agency

　　

　　Choosing a reputable maid agency registered with the Ministry of Manpower (MOM) can simplify the hiring process. Agencies handle much of the paperwork and can provide candidates that match your requirements. When selecting an agency, consider the following:

　　

　　- Reputation: Research the agency¨s track record and read reviews from other employers.

　　

　　- Services Offered: Ensure the agency provides comprehensive services, including medical check-ups, training, and documentation assistance.

　　

　　- Fees: Understand the agency¨s fee structure and any additional costs involved.

　　

　　4. Interview Potential Helpers

　　

　　Conducting interviews is a crucial step to ensure you find a suitable domestic helper. Prepare a list of questions to assess the candidate¨s experience, skills, and compatibility with your family. Some important aspects to consider during the interview include:

　　

　　- Work Experience: Ask about their previous employment, specific tasks they handled, and the duration of their employment.

　　

　　- Skills: Evaluate their proficiency in tasks relevant to your needs, such as cooking, cleaning, or childcare.

　　

　　- Language Proficiency: Communication is key, so ensure the candidate has a reasonable command of a language you and your family are comfortable with.

　　

　　- Personality and Compatibility: Consider whether the candidate¨s personality and values align with your family¨s dynamics.

　　

　　5. Medical Examination

　　

　　Before hiring, ensure the candidate undergoes a medical examination to confirm they are fit for work. This is a mandatory requirement by MOM. The medical examination typically includes:

　　

　　- Health Screening: General health assessment to check for any pre-existing conditions.

　　

　　- Infectious Disease Tests: Tests for infectious diseases such as tuberculosis, HIV, and hepatitis.

　　

　　6. Apply for a Work Permit

　　

　　Once you¨ve selected a candidate, the next step is to apply for a work permit. This can be done through MOM¨s website. The application process involves:

　　

　　- Filling out the Application Form: Provide necessary details about yourself and the helper.

　　

　　- Paying the Application Fee: The fee varies and is payable online.

　　

　　7. Purchase Medical Insurance and a Security Bond

　　

　　It¨s mandatory to purchase medical insurance and a security bond for the helper.

　　

　　- Medical Insurance: Coverage should be at least SGD 15,000 per year for inpatient care and day surgery. This ensures the helper has access to medical care if needed. [Please refer to MOM website for the latest required coverage amount LINK” target=_blank>

　　

　　- Security Bond: A financial guarantee to the Singapore government, usually SGD 5,000 for non-Malaysian helpers. This bond can be in the form of a banker¨s guarantee or insurance.

　　

　　8. Orientation Program

　　

　　Both you and your helper must attend the Settling-In Programme (SIP) and Employers’ Orientation Programme (EOP) [LINK” target=_blank> . The SIP is a one-day course designed to educate first-time helpers and employers on safety, rights, and responsibilities. The program includes:

　　

　　- Safety Training: Teaching helpers about safe working practices and accident prevention.

　　

　　- Rights and Responsibilities: Educating both parties on their legal rights and responsibilities.

　　

　　- Cultural Orientation: Helping helpers adapt to the cultural norms and practices in Singapore.

　　

　　9. Arrival and Settling In

　　

　　Once all the documentation is in place, arrange for the helper¨s arrival in Singapore. Upon arrival, it¨s important to provide a smooth settling-in process. Consider the following:

　　

　　- Orientation: Introduce the helper to your home and the surrounding area. Explain household routines and rules.

　　

　　- Expectations: Clearly outline the helper¨s duties, working hours, and rest days. Establish open communication to address any concerns or questions.

　　

　　- Support: Provide the helper with necessary supplies, such as toiletries and work-related tools. Offer assistance in adapting to the new environment.

　　

　　10. Ongoing Management

　　

　　Maintaining a positive working relationship with your helper requires ongoing management. Here are some tips:

　　

　　- Regular Communication: Keep an open line of communication to address any issues or misunderstandings promptly.

　　

　　- Fair Treatment: Treat your helper with respect and fairness. Ensure they have adequate rest, reasonable working hours, and proper living conditions.

　　

　　- Compliance with Regulations: Stay informed about MOM regulations and ensure compliance. This includes timely renewal of work permits, paying the monthly levy, and providing medical insurance.

　　

　　Necessary Documentation and Legal Requirements

　　

　　1. Work Permit Application

　　

　　The work permit application involves several documents and steps:

　　

　　- Completed Application Form: Available on MOM¨s website.

　　

　　- Copy of the Helper¨s Passport: Ensure it¨s valid for at least 7 months.

　　

　　- Employment Contract: A contract signed by both parties outlining the terms of employment, salary, rest days, and other conditions.

　　

　　- Medical Examination Report: A report confirming the helper is fit for work.

　　

　　2. Medical Insurance

　　

　　You must purchase medical insurance that covers at least SGD 15,000 per year for inpatient care and day surgery. This ensures that the helper has access to necessary medical care without financial strain on either party.

　　

　　3. Security Bond

　　

　　A security bond of SGD 5,000 is required for non-Malaysian helpers. This bond acts as a financial guarantee to the Singapore government. It can be in the form of a banker¨s guarantee or insurance. The security bond ensures compliance with MOM regulations and covers any potential repatriation costs if the helper breaches the terms of employment.

　　

　　4. Settling-In Programme (SIP)

　　

　　The Settling-In Programme is mandatory for first-time helpers and employers. The one-day course includes:

　　

　　- Safety and Health Practices: Training on workplace safety and health.

　　

　　- Rights and Responsibilities: Information on the legal rights and responsibilities of both employers and helpers.

　　

　　- Cultural Adaptation: Guidance on adapting to the cultural norms and practices in Singapore.

　　

　　5. Monthly Levy

　　

　　Employers are required to pay a monthly levy to MOM. The levy rates vary based on the type of household and whether the helper is caring for children, elderly, or disabled family members. Levy rates are typically lower for households with dependents who need care.

　　

　　6. Employer¨s Orientation Programme (EOP)

　　

　　First-time employers must attend the Employer¨s Orientation Programme. This program can be completed online or in person and covers:

　　

　　- Responsibilities as an Employer: Understanding your obligations and responsibilities as an employer.

　　

　　- Employment Regulations: Information on MOM regulations and how to comply with them.

　　

　　- Conflict Resolution: Strategies for managing conflicts and maintaining a positive working relationship with your helper.

　　

　　7. Employment Contract

　　

　　An employment contract is a legally binding document that outlines the terms of employment. It should include:

　　

　　- Duties and Responsibilities: A detailed list of the helper¨s duties and responsibilities.

　　

　　- Salary: The agreed monthly salary and any additional allowances.

　　

　　- Rest Days: The number of rest days per month and compensation for working on rest days.

　　

　　- Working Hours: The expected working hours and any overtime policies.

　　

　　- Termination Clause: Conditions under which the employment contract can be terminated by either party.

　　

　　8. Rest Days and Overtime

　　

　　Helpers are entitled to at least one rest day per week. If the helper agrees to work on a rest day, they must be compensated with extra pay or given another rest day in lieu. Ensure that the employment contract clearly outlines the rest day policy and compensation for overtime work.

　　

　　Conclusion

　　

　　Hiring a domestic helper in Singapore involves several steps and adherence to legal requirements. By following this comprehensive guide and ensuring all necessary documentation is in place, you can smoothly navigate the hiring process and establish a positive working relationship with your domestic helper.

　　

　　Remember, a harmonious employer-helper relationship is built on mutual respect, clear communication, and compliance with regulations. Taking the time to understand and fulfill your responsibilities as an employer will create a positive environment for both you and your helper.

　　

　　—

　　

　　Feel free to ask if you need more details or have specific questions about any part of the process!

　　This blog post will guide you through the factors to consider when selecting a domestic helper and provide interview tips and questions to ask to ensure you make the best choice for your family.In the long run, Singapore house maid agency The value will be higher and higher, and there will be a great leap in essence. https://1achieve.net

　　

　　ESSENTIAL KNOWLEDGE

　　

　　Bryan

　　

　　8/3/20246 min read

　　

　　Factors to Consider When Selecting a Domestic Helper

　　

　　1. Experience and Skills

　　

　　Consider the domestic helper¨s experience and skills relevant to your household needs. This includes:

　　

　　Childcare: If you have young children, look for a helper with experience in childcare, including feeding, bathing, and educational activities. A helper with specific experience in handling infants, toddlers, or school-aged children can be particularly beneficial. Inquire about their familiarity with safety protocols, such as CPR and first aid, to ensure they can handle emergencies.

　　

　　Elderly Care: For households with elderly members, a helper with experience in elder care, such as assisting with mobility, medication, and companionship, is essential. Helpers trained in handling dementia or chronic illnesses can provide specialized care that significantly improves the quality of life for elderly family members.

　　

　　Housekeeping: General housekeeping skills, including cleaning, laundry, and cooking, are vital for maintaining a tidy and organized home. Assess their expertise in using modern appliances, knowledge of cleaning techniques, and ability to cook various cuisines if that is important to your household.

　　

　　Language Proficiency: Communication is key, so consider a helper who can speak and understand the language(s) spoken in your household. This ensures smooth communication and reduces the likelihood of misunderstandings.

　　

　　2. Personality and Compatibility

　　

　　A domestic helper¨s personality and compatibility with your family are crucial for a harmonious household. Look for traits such as:

　　

　　Patience: Especially important for childcare and elderly care. A patient helper can manage stressful situations calmly and effectively, ensuring the well-being of vulnerable family members.

　　

　　Adaptability: The ability to adjust to new environments and routines. An adaptable helper can quickly learn and integrate into your household¨s unique dynamics, contributing to a smoother transition.

　　

　　Honesty and Trustworthiness: Essential for the safety and security of your household. Reliable helpers build a foundation of trust, making it easier for you to delegate responsibilities with confidence.

　　

　　Work Ethic: A strong sense of responsibility and diligence in performing tasks. Look for a helper who demonstrates a proactive approach to their duties and takes pride in their work.

　　

　　3. Cultural Background and Dietary Preferences

　　

　　Consider the cultural background and dietary preferences of the helper, as these can influence their comfort and effectiveness in your household. For example:

　　

　　Cultural Practices: Some helpers may have specific cultural practices that need to be respected, such as religious observances. Understanding and accommodating these practices fosters a respectful and inclusive environment.

　　

　　Dietary Restrictions: Be aware of any dietary restrictions or preferences, which may affect meal preparation and household routines. A helper who shares similar dietary habits can easily integrate into your family¨s meal planning and cooking routines.

　　

　　4. Health and Medical History

　　

　　The health and medical history of the helper are important to ensure they can perform their duties effectively and safely. Consider:

　　

　　Medical Fitness: Ensure the helper is medically fit to perform physical tasks. This includes a thorough medical examination to rule out any conditions that might hinder their ability to work.

　　

　　Previous Medical Conditions: Be aware of any chronic conditions that may require special attention or affect their ability to work. Understanding their medical history helps you prepare for any potential health-related issues.

　　

　　5. References and Background Checks

　　

　　Conducting thorough reference and background checks can provide insight into the helper¨s past performance and reliability. Consider:

　　

　　References: Ask for and verify references from previous employers. Inquire about the helper¨s performance, reliability, and any challenges they faced.

　　

　　Background Checks: Conduct a background check to ensure there are no criminal records or other red flags. This provides an added layer of security and peace of mind.

　　

　　6. Agency Reputation

　　

　　If you are hiring through an agency, ensure that the agency is reputable and provides comprehensive support services, such as:

　　

　　Training Programs: Agencies that offer training programs for helpers can ensure they are well-prepared for their duties. Look for agencies that provide training in childcare, elder care, housekeeping, and emergency response.

　　

　　Support Services: Agencies that provide ongoing support, including conflict resolution and replacement services, can be very helpful. This ensures you have a reliable partner to turn to if any issues arise during the employment period.

　　

　　7. Legal and Regulatory Compliance

　　

　　Ensure that the hiring process complies with all legal and regulatory requirements in your country. This includes:

　　

　　Work Permits and Visas: Verify that the helper has the necessary work permits and visas to work legally in your country.

　　

　　Contracts and Agreements: Ensure that the employment contract adheres to local labor laws and includes all necessary terms and conditions, such as salary, working hours, rest days, and termination clauses.

　　

　　8. Financial Considerations

　　

　　Consider the financial implications of hiring a domestic helper. This includes:

　　

　　Salary: Determine a fair and competitive salary based on market rates and the helper¨s experience and skills. Ensure the salary meets or exceeds the legal minimum wage.

　　

　　Additional Costs: Factor in additional costs such as agency fees, work permit fees, insurance, and medical expenses.

　　

　　9. Personal and Professional Boundaries

　　

　　Establish clear personal and professional boundaries to ensure a respectful and professional working relationship. This includes:

　　

　　Privacy: Respect the helper¨s privacy and personal space, and ensure they have a comfortable living environment.

　　

　　Communication: Maintain open and respectful communication to address any concerns or issues that may arise.

　　

　　10. Trial Period

　　

　　Consider implementing a trial period to assess the helper¨s compatibility and performance before committing to a long-term contract. This allows both parties to determine if the arrangement is a good fit.

　　

　　Interview Tips and Questions to Ask

　　

　　The interview process is critical in assessing whether a domestic helper is the right fit for your family. Here are some tips and questions to guide you:

　　

　　1. Prepare a List of Questions

　　

　　Having a prepared list of questions ensures you cover all important topics. Some key questions include:

　　

　　Experience and Skills:

　　

　　＾Can you describe your previous experience as a domestic helper?￣

　　

　　＾What specific tasks did you perform in your previous jobs?￣

　　

　　＾Are you comfortable taking care of young children/elderly family members?￣

　　

　　Personality and Compatibility:

　　

　　＾How do you handle stress or difficult situations?￣

　　

　　＾What do you enjoy most about being a domestic helper?￣

　　

　　＾How do you prefer to communicate with your employer?￣

　　

　　Cultural Background and Dietary Preferences:

　　

　　＾Are there any cultural practices or dietary restrictions we should be aware of?￣

　　

　　＾How do you handle cooking meals with dietary restrictions?￣

　　

　　Health and Medical History:

　　

　　＾Do you have any medical conditions or allergies we should know about?￣

　　

　　＾Are you comfortable with the physical demands of this job?￣

　　

　　Availability and Commitment:

　　

　　＾When are you available to start?￣

　　

　　＾Are you willing to commit to a long-term employment contract?￣

　　

　　2. Observe Non-Verbal Cues

　　

　　Pay attention to the helper¨s body language, eye contact, and overall demeanor during the interview. These cues can provide additional insights into their confidence, honesty, and attitude.

　　

　　3. Discuss Specific Scenarios

　　

　　Presenting specific scenarios can help you understand how the helper would handle various situations. For example:

　　

　　＾What would you do if my child refuses to eat their meal?￣

　　

　　＾How would you handle an emergency, such as a fire or medical situation?￣

　　

　　＾Can you give an example of how you resolved a conflict with a previous employer?￣

　　

　　4. Involve Family Members

　　

　　Involving family members, especially those who will interact closely with the helper, can provide additional perspectives. This is particularly important if the helper will be caring for children or elderly family members.

　　

　　5. Clarify Expectations and Boundaries

　　

　　Clearly communicate your expectations, household rules, and boundaries during the interview. This includes:

　　

　　Work Hours: Outline the expected working hours and any flexibility required.

　　

　　Rest Days: Discuss the helper¨s rest days and how they will be compensated for any additional work.

　　

　　Privacy and Respect: Explain the importance of privacy and respect within your household.

　　

　　6. Discuss Compensation and Benefits

　　

　　Discuss the helper¨s salary, benefits, and any additional perks you may offer. This includes:

　　

　　Salary: Ensure the salary meets the legal minimum and is fair for the work expected.

　　

　　Benefits: Discuss any additional benefits, such as medical coverage, bonuses, or training opportunities.

　　

　　Living Arrangements: If the helper will live with your family, explain the living arrangements and amenities provided.

　　

　　7. Assess Willingness to Learn

　　

　　A helper¨s willingness to learn and adapt is crucial for long-term success. Ask questions such as:

　　

　　＾Are you open to learning new skills or methods of doing tasks?￣

　　

　　＾How do you feel about attending training programs or workshops?￣

　　

　　8. Trust Your Instincts

　　

　　Finally, trust your instincts. If something feels off during the interview, it¨s important to consider that feeling in your decision

　　The Key to a Harmonious Employer-Domestic Helper Relationship in SingaporeAs we all know, maid agency Singapore The emergence of the market is worthy of many people’s attention, which has aroused the waves of the whole market. https://1achieve.net

　　

　　ESSENTIAL KNOWLEDGE

　　

　　Bryan

　　

　　7/2/20243 min read

　　

　　1. Importance of Effective Communication

　　

　　Imagine a household where instructions are understood the first time they’re given, where concerns are expressed and addressed promptly, and where laughter often fills the air because jokes and stories are shared and appreciated. This isn’t a utopian dream but a realistic scenario in homes where clear communication prevails between employers and their domestic helpers.Effective communication fosters a sense of trust and respect. It minimizes misunderstandings and conflicts, making daily interactions smoother and more pleasant. According to Dr. Sarah Tan, a family psychologist, “Clear communication is the bedrock of any strong relationship. In the context of employers and domestic helpers, it’s not just about conveying tasks; it’s about building a mutual understanding and respect that transcends cultural and linguistic barriers.”

　　

　　2. Tips for Language Learning

　　

　　For employers and domestic helpers eager to bridge the language divide, here are some actionable tips to enhance language skills:

　　

　　Leverage Technology: Use language learning apps like Duolingo or Babbel, which offer interactive lessons on various languages.

　　

　　Practice Daily: Encourage daily conversations, no matter how basic, to build confidence and fluency.

　　

　　Language Exchange: Set aside time each week for a language exchange session where both parties teach each other their native languages.

　　

　　Utilize Media: Watch movies, listen to music, or read books in the language being learned to familiarize with its usage in different contexts.

　　

　　Real-life story: Maria, a domestic helper from the Philippines, shared, “My employer and I dedicated 30 minutes every evening to teach each other our languages. Not only has my English improved, but we’ve also grown closer through the process.”

　　

　　3. Overcoming Language Barriers

　　

　　Despite best efforts, language barriers can persist. Here are some strategies and technologies to help bridge these gaps:

　　

　　Translation Apps: Tools like Google Translate can facilitate immediate translation and understanding.

　　

　　Pictorial Aids: Use pictures or symbols for common tasks or items around the house to avoid confusion.

　　

　　Non-verbal Communication: Sometimes, actions speak louder than words. Gestures, facial expressions, and body language can convey messages effectively.

　　

　　Expert quote: “In today’s digital age, technology offers unprecedented ways to overcome language barriers,” says tech analyst Jason Lim. “However, the willingness to understand and be patient with each other is equally important.”

　　

　　4. Cultural Nuances in Communication

　　

　　Understanding the cultural context of communication can significantly enhance interactions. For instance, in some cultures, direct eye contact might be considered rude, while in others, it’s a sign of honesty.

　　

　　Employers and domestic helpers should take the time to learn about each other’s cultural backgrounds. This knowledge can prevent unintentional offenses and deepen mutual respect.

　　

　　Testimonial: Aishah, an employer, noted, “Learning about my helper’s cultural background helped me understand her better. It was enlightening to see how our communication improved as we became more culturally aware.”

　　

　　5. Building a Positive Relationship Through Communication

　　

　　Open and respectful dialogue is the foundation of a positive relationship. Here are some practices to encourage:

　　

　　Regular Check-ins: Have weekly meetings to discuss any concerns or suggestions.

　　

　　Active Listening: Show genuine interest in understanding the other person’s perspective.

　　

　　Express Appreciation: Acknowledge each other’s efforts and express gratitude regularly.

　　

　　Engaging question: How can you implement these communication strategies in your household to foster a stronger bond with your domestic helper?

　　

　　Call to action: Start today by choosing one tip from this article to improve communication with your domestic helper. Remember, small steps can lead to significant changes in building a harmonious and respectful relationship.

　　

　　In conclusion, language and communication abilities are crucial in ensuring a smooth and positive relationship between employers and domestic helpers in Singapore. By prioritizing effective communication, embracing language learning, overcoming barriers with technology, understanding cultural nuances, and fostering open dialogue, employers and domestic helpers can create a nurturing and respectful home environment.

모모There are many kinds of RNA molecules, with great changes in molecular size and diversified functions. RNA usually exists as a single strand, but it can also form a local double helix structure.More importantly, put small molecules It is imperative for us to make thorough analysis and maximize its social function. https://www.all-chemistry.com/

모모

모모1. Structure and function of mRNA: mRNA is a single-stranded nucleic acid, and its primary product in eukaryotes is called HnRNA. Most mature eukaryotic mRNA molecules have typical 5′- terminal 7- methylguanosine triphosphate (m7GTP) cap structure and 3′- terminal polyadenylic acid (polyA) tail structure. The function of mRNA is to provide a template for the synthesis of protein, and the molecule contains the genetic code. Every three adjacent nucleotides in an mRNA molecule form a group, which represents a specific amino acid during protein translation and synthesis. This nucleotide triplet is called the genetic code (coden).

모모

모모2. Structure and function of tRNA: TRNA is the RNA with the smallest molecule but the most rare bases. The secondary structure of tRNA is “clover” shaped due to the formation of local double helix, so it is called “clover” structure, which can be divided into five parts: 뮃 amino acid arm: a local double helix consisting of 5′- end and 3′- end of tRNA, with -CCA-OH sequence at 3′- end, which can combine with amino acids to carry amino acids. 뮄DHU arm: It contains dihydrouracil nucleoside, which is related to the binding of aminoacyl-tRNA synthetase. 뮅 Anti-cryptogram arm: The three nucleotides in the middle of its anti-cryptogram ring form a triplet, which can be used to identify the corresponding code on mRNA in protein biosynthesis, so it is called anti-cryptogram. 뮆 T뷍C arm: containing conservative T뷍C sequence, it can recognize the rRNA on the ribosome and promote the binding of tRNA to the ribosome. 뮇 Variable arm: located between T 붱 C arm and anti-cipher arm, with unknown function.

모모

모모3. Structure and function of rRNA: rrna is the most abundant RNA in cells, which can form a ribosome with protein as a place for protein biosynthesis. There are three kinds of rRNA in prokaryotes: 5S, 16S and 23S. There are four kinds of rRNA in eukaryotes: 5S, 5.8S, 18S and 28S.

　　Protein is a substance with a certain spatial structure, which is formed by the zigzag folding of polypeptide chains composed of amino acids in the form of dehydration and condensation. Protein must contain carbon, hydrogen, oxygen and nitrogen.From the demand side, recombinant proteins More in line with the psychological expectations of consumers, willing to pay for the things they like. https://www.alphalifetech.com/

　　

　　Protein is a polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Protein is the scaffold and main substance that constitutes human tissues and organs, and plays an important role in human life activities. It can be said that there would be no life activities without protein.

　　

　　Number of atoms

　　

　　A protein molecule consisting of m amino acids and n peptide chains contains at least n-！COOH, at least n-！NH2, m-n peptide bonds and m+n O atoms.

　　

　　molecular mass

　　

　　Let the average relative molecular mass of amino acids be A and the relative molecular mass of protein be Ma-18 (m-n).

　　

　　Gene control

　　

　　Nucleotide 6 in the gene

　　

　　Nucleotide 3 in messenger RNA

　　

　　Amino acid 1 in protein

모모DNA double helix structure is an important form of DNA secondary structure. It is a structural model put forward by Watson and Crick in 1953.know inhibitors The market will definitely bring great influence to the whole industry. https://www.all-chemistry.com/

모모

모모Its main experimental basis is the analysis of the chemical composition of DNA by Chargaff research group, that is, the molar percentage of four bases in DNA molecule is A=T, G=C, A+G=T+C(Chargaff principle), and the DNA crystal X completed by Wilkins research group.

모모

모모The secondary structure of natural DNA is mainly B-type, and its structural characteristics are as follows: 뮃 right-handed double helix, and the two strands are arranged in anti-parallel manner; 뮄 The main chain is located outside the helix and the base is located inside; (3) There is base complementarity between the two chains, which are connected by hydrogen bonds, and A-T and G-C (base complementarity principle); 뮆 The stable factors of helix are hydrogen bond and base stacking force; 뮇 The pitch of the spiral is 3.4nm and the diameter is 2nm.

　　Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen). Generally, protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).In the industry, Antibody Phage Display Service Has been a leader in the industry, but later came from behind but never arrogant, low-key to adhere to quality. https://www.alphalifetech.com/

　　

　　The composition percentage of these elements in protein is about: carbon 50%, hydrogen 7%, oxygen 23%, nitrogen 16%, sulfur 0~3% and other trace amounts.

　　

　　(1) All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

　　

　　(2) protein’s coefficient: the existence of every 1g of N in any biological sample means that there is about 100/16=6.25g of protein, and 6.25 is often called protein’s constant.

　　

　　(3) protein is a biopolymer based on amino acids. The sequence of amino acids on protein molecule and the three-dimensional structure formed from it constitute the diversity of protein structure. Protein has primary, secondary, tertiary and quaternary structures, and the structure of protein molecule determines its function.

　　Classification of amino acids, several special amino acids, molecular structure and physical and chemical properties of protein, composition of nucleic acid, double helix structure of DNA, basic concept of enzyme, Michaelis equation, coenzyme composition.To some extent, inhibitors Our development has surpassed many peer businesses, but it has never stopped moving forward. https://www.all-chemistry.com/

　　

　　Memorize 20 kinds of amino acids and try to remember English abbreviations and codes as much as possible, because exams often appear directly with codes. Protein’s molecular structure often examines the manifestations of various levels of structure and their bonds. The physical and chemical properties of protein and the purification of protein usually use the physical and chemical properties of protein to purify protein without destroying the structure of protein. Pay attention to the identification of amino acids and physical and chemical properties of protein. The basic unit of nucleic acid is nucleotide, which is composed of multiple nucleotides, and the connecting bond between nucleotides is 3′, 5′- phosphodiester bond. DNA double helix structure, in which two bases exist in strict accordance with A = T (two hydrogen bonds) and G triple C (three hydrogen bonds), and the characteristics of various RNA.

　　

　　In addition, we should pay attention to some commonly used concepts in nucleic acid problem solving. The first thing that enzymes should pay attention to is some basic concepts, such as ribozyme, deoxyribozyme, enzyme activity center, isozyme, isomerase and so on. The key point of the Metric Equation test is V=Vmax[S” target=_blank>/Km+[S” target=_blank>, which explains the equation of the relationship between the concentration of enzymatic reaction and the reaction speed. In the exam, candidates are sometimes asked to do simple calculations according to this equation before they can answer. The difference of several inhibitors.

　　

　　According to the characteristics of allosteric enzymes, it should be noted that allosteric regulation can cause conformational changes of enzymes. It is important to pay special attention here that configuration refers to the basic structural composition of substances, and conformation refers to the spatial change of substances. Allosteric regulation can cause the conformational change of enzymes, but not the conformational change of enzymes.

　　Protein is a complex organic polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Amino acids are the basic units of protein, and they are linked into peptide chains through dehydration and condensation. Each polypeptide chain has twenty to hundreds of amino acid residues (-R); Various amino acid residues are arranged in a certain order.We have every reason to believe. Antibody Discovery It will become the mainstream of the industry and will gradually affect more and more people. https://www.alphalifetech.com/

　　

　　the composition characteristics of protein

　　

　　The amino acid sequence of protein is encoded by the corresponding gene. In addition to the 20 basic amino acids encoded by the genetic code, in protein, some amino acid residues can be modified after translation to change their chemical structure, thus activating or regulating protein. Multiple protein can form a stable protein complex together, often by combining together, and fold or spiral to form a certain spatial structure, thus playing a specific function. The organelles of synthetic peptides are ribosomes on rough endoplasmic reticulum in cytoplasm. The difference of protein lies in the variety, number, arrangement order of amino acids and the spatial structure of peptide chains.

　　

　　Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen), and generally protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).

　　

　　The composition percentage of these elements in protein is about: 50% carbon, 7% hydrogen, 23% oxygen, 16% nitrogen, 0-3% sulfur and other trace amounts.

　　

　　All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

　　

　　Every 1g of N in any biological sample means that there is about 100/16=6.25g protein, and 6.25 is often called protein constant.

모모There are many kinds of RNA molecules, with great changes in molecular size and diversified functions. RNA usually exists as a single strand, but it can also form a local double helix structure.However, with the development of the industry, small molecules It will also bring us more and more consumer experiences, so that users can really feel the upgrade and change. https://www.all-chemistry.com/

모모

모모1. Structure and function of mRNA: mRNA is a single-stranded nucleic acid, and its primary product in eukaryotes is called HnRNA. Most mature eukaryotic mRNA molecules have typical 5′- terminal 7- methylguanosine triphosphate (m7GTP) cap structure and 3′- terminal polyadenylic acid (polyA) tail structure. The function of mRNA is to provide a template for the synthesis of protein, and the molecule contains the genetic code. Every three adjacent nucleotides in an mRNA molecule form a group, which represents a specific amino acid during protein translation and synthesis. This nucleotide triplet is called the genetic code (coden).

모모

모모2. Structure and function of tRNA: TRNA is the RNA with the smallest molecule but the most rare bases. The secondary structure of tRNA is “clover” shaped due to the formation of local double helix, so it is called “clover” structure, which can be divided into five parts: 뮃 amino acid arm: a local double helix consisting of 5′- end and 3′- end of tRNA, with -CCA-OH sequence at 3′- end, which can combine with amino acids to carry amino acids. 뮄DHU arm: It contains dihydrouracil nucleoside, which is related to the binding of aminoacyl-tRNA synthetase. 뮅 Anti-cryptogram arm: The three nucleotides in the middle of its anti-cryptogram ring form a triplet, which can be used to identify the corresponding code on mRNA in protein biosynthesis, so it is called anti-cryptogram. 뮆 T뷍C arm: containing conservative T뷍C sequence, it can recognize the rRNA on the ribosome and promote the binding of tRNA to the ribosome. 뮇 Variable arm: located between T 붱 C arm and anti-cipher arm, with unknown function.

모모

모모3. Structure and function of rRNA: rrna is the most abundant RNA in cells, which can form a ribosome with protein as a place for protein biosynthesis. There are three kinds of rRNA in prokaryotes: 5S, 16S and 23S. There are four kinds of rRNA in eukaryotes: 5S, 5.8S, 18S and 28S.